human nt2 d1  (ATCC)

Journal: bioRxiv

Article Title: Investigating the role of melatonin in bipolar disorder using transcriptomics

doi: 10.1101/2025.09.03.673928

Figure Lengend Snippet: The post-mortem bipolar disorder (BD) gene expression dataset was created using RNA sequencing (RNAseq) data from post-mortem dorsolateral prefrontal cortex (DLPFC) samples obtained from BD patients and healthy controls (HC). This dataset was used to create gene regulatory networks, which were then compared to a list of genes related to melatonin synthesis, signalling, and degradation using gene set enrichment analysis (GSEA), and to gene regulatory networks for melatonin agonists. The NT2-N gene expression dataset was created using RNAseq libraries prepared from differentiated NT2-N neurons treated with various mood stabilisers for 24 hours. This dataset was then compared to a list of melatonin-related genes using GSEA. [Created by authors with biorender.com ]

Article Snippet: This dataset consists of RNAseq data collected from human NT2 teratocarcinoma cells (ATCC, United States; RRID: CVCL_0034).

Techniques: Gene Expression, RNA Sequencing

Journal: Cellular & Molecular Biology Letters

Article Title: NT2-derived astrocyte–neuron co-culture reflects physiological relevance and offers research validity

doi: 10.1186/s11658-025-00765-z

Figure Lengend Snippet: A Representative morphological features of Neu (neuron-enriched), eA (early astrocytes), and mA (mature astrocytes) cultures. Scale bars always indicate 50 µm. B Immunocytochemical staining for neuronal and astrocytic markers. Each tile scan is composed of 20 images. Supplementary Fig. S5A shows the images without zoomed inserts, while Supplementary Fig. S5B presents additional images of neuronal staining in mA cultures. Scale bars always indicate 100 µm. C Time-dependent changes in the GFAP level upon astrocyte maturation monitored using Western blot with densitometry signals for GFAP normalized to the total protein load per well, as determined by ruthenium staining, with available Supplementary Data F1B and Supplementary Fig. S4. w0 denotes the starting point of maturation after the completion of retinoic acid treatment. D The distribution of cell diameters for eA and mA relative to undifferentiated NT2 cells, with n = 25, was calculated using ImageJ software, assuming a circular cell shape. Owing to variance inequality, the Kruskal–Wallis test was used for statistical analysis

Article Snippet: Human pluripotent NT2 cells (ATCC; NTERA-2 cl.D1 cell line, ATCC-CRL-1973) were maintained as previously described [ – ], with certain modifications.

Techniques: Staining, Western Blot, Software

Journal: Cellular & Molecular Biology Letters

Article Title: NT2-derived astrocyte–neuron co-culture reflects physiological relevance and offers research validity

doi: 10.1186/s11658-025-00765-z

Figure Lengend Snippet: A Principal component analysis (PCA) of proteomic data [log 2 label-free quantification (LFQ)] for the studied cell types. The PCA plot represents 3827 proteins (meeting the condition of having LFQ values present in 70% of all samples) with biological replicates, indicating clear proteomic profile differences between Neu (neuron-enriched), eA (early astrocytes), mA (mature astrocytes), and NT2 (undifferentiated) cells. B Hierarchical clustering and a heatmap. The 2145 proteins whose amount significantly differed between the four cell types [ANOVA permutation based false discovery rate (FDR) < 0.0001] are represented in the heatmap. C Volcano plots of the differentially expressed proteins (DEPs). The red and blue dots represent significantly upregulated and downregulated DEPs, respectively. The horizontal dotted line represents −log 10 ANOVA p -value ≥ 4; vertical dashed lines indicate fold change |log 2 FC|≥ 1

Article Snippet: Human pluripotent NT2 cells (ATCC; NTERA-2 cl.D1 cell line, ATCC-CRL-1973) were maintained as previously described [ – ], with certain modifications.

Techniques: Quantitative Proteomics